In the tunnel

I was recently informed by one of my thesis committee members that I am IN THE TUNNEL meaning that completion of the PhD is near. YES!!!!

With that said, I am writing a manuscript and considering where to pursue a postdoctoral fellowship. I am SO excited to be doing these things.... but I feel a little anxious about the next stages of my career, namely finding that postdoctoral research position.

Finding the right lab to conduct postdoctoral research is extremely crucial to the advancement of my career. Everything must be considered, including (in no particular order of importance because it's all important):

• Research focus (because whatever I choose to study at this stage will define my career, right?)
• Geographical location (where do I want to spend the next 5+ years of my life?)
• Mentoring capabilities...What type of mentoring style (hands-on or hands-off) does the principal investigator have?
• What are the social dynamics of the lab?
• What type of grants/funding does the PI have?
• How successful were past trainees of the potential PI? (where and how often did they publish, what kind of position did they obtain after leaving?)
• Big lab or small lab?
• Well-known or up-and-coming mentor?
• What type of collaborations does the mentor have with other labs or industry?

There are always other things to consider, so did I leave anything out??

For now, I am narrowing down the labs that I am interested at a few different universities and based on the criteria specified above.

Singing the lab blues

I am having some difficulties with my project. I am in some kind of slump in which none of my experiments are working. I mean the simple PCR reaction is not working.  The cells are not proliferating fast enough. And on top of everything, I placed an order with the person in the lab who is responsible for ordering reagents and due to no fault of his own, the order did not go through. It turns out there was some kind of computer glitch that day and the email to place the order never was received by the company, but I found this out like two weeks after I requested the reagent to be ordered.  (And I thought that I gave myself enough cushion room for this by placing the order before I ran out of the supply I had in the lab.)  So, that experiment is on hold for at least a month because the reagent is made-to-order.  To top everything off, I am not getting any attention from my PI because I do not have any NEW data. Sigh. I need some encouraging words because right now, it is all bad.

Thank Goodness It's Friday???, Part II

In an earlier post, I described how my Friday would be hectic, to say the least. I also said that I would let you know how my Friday turned out. Well, yes, it was a whirlwind day. The lab meeting w e n t.  I did not make it to my networking event as an unexpected visitor came to our lab. But this was okay because he was an old lab-mate who stopped by to say hello and have lunch with us. Once this was over, I should have turned my focus to my presentation, B U T no... I was fussing over some old data.  Finally at about 1:30pm, I turned my attention to my presentation (which I presented once before) and began rehearsing it to myself.

3PM: SHOWTIME! As I began to speak, I felt my heart race, as it does whenever I give a talk. I mean, I know my research and what I want to say, but it's just a will of nerves hence, rapid heartbeat. (I think the nerves come in because I am wondering what the audience is thinking of me. Am I good enough? How is my data? Is my hair sticking up? Am I slouching? Am I speaking loud enough? Do they care about what I am saying? OMG is that person SLEEP?!) Then there were some technical difficulties which garnered a few laughs from the audience which was nearly half-dead from the intense interviews with professors earlier in the day. But their laughter eased my nerves.

Mid-way through the talk, I glanced at the clock and realized that I had only 2min left, since I was trying to speak slowly (I have been told that I talk too fast), so I rushed the last half.  When it was over, I knew that some of the students were actually paying attention because 3 asked me questions.

SELF ASSESSMENT I have critiques of my presentation: 1) I stumbled with words here and there. It's like I am trying to say one thing and already thinking about the next thing I want to say and what comes out my mouth is a jumbled mess of the first and second thoughts. Speaking slower will correct this issue. 2) I notice that I have the tendency to "talk to myself" while presenting. For example, I think there was a point that I said out loud, "oh no that is not right..." in reference to the order I wanted to say something. And I was on a microphone, sigh.  Although I know this presentation was not perfect, I can feel myself growing and becoming better. I am aware of some of my flaws and approach others to learn of other flaws so that I can work on correcting them.  I have another event at the end of the month. Between now and then, all I can do is practice, practice and practice so more.

Oh yes, I only managed to get a minimal amount of lab work done on Friday. So, now I am rushing my daughter to "get it together" and get dressed so that we can spend a Saturday in the lab. I know she is not happy with me about this. But it's the way it is. Maybe we will go for ice cream later. Geez, I feel like I am bribing the girl, sigh.

Thank Goodness It's Friday???

Ah, tomorrow is Friday. I should be excited that it is the last day of the week and the weekend is almost here. Uh, no.  My Friday is crammed. I have to give the lab meeting tomorrow at 9am. (And yes, after making some easy-to-fix cloning mistakes, I did get the data I mentioned in an earlier post.) I committed to attending a networking event at noon. I jumped at the opportunity to give a short talk about my research to recruits for my graduate program at 3pm.  I will play school bus and shuttle my daughter from her elementary school to her ballet school before 4pm and then pick her up by 5:30pm. Somewhere in all this chaos, I will get a few experiments done. Oh yeah, I will eat at some point. This is my life, my life as a single parent pursuing a PhD. No complaints, just feeling a wee bit stressed. And oh yeah, tomorrow night will be a Papa John's night! I will let you know how things go.

Time for the lab report

Before I could enjoy my morning coffee I had to throw on some sweats, dress my daughter and dart into the lab to setup an experiment that will hopefully support one of my many hypotheses.  I am in a time crunch because I really want to generate (good) data before I have to give lab meeting in two weeks.  What does "give lab meeting" mean? Well, every week my thesis lab gathers together to hold a lab meeting.  During this lab meeting, one member of the lab will present his/her data to the rest of the lab members, including our PI (Principle Investigator aka "the boss.") The presentation is typically in the form of PowerPoint slides and lasts for about one hour.  During the presentation, the presenter shares the rationale, methods and results for his/her recent experiments. If s/he encountered any problems with the experiment, we usually try to aid the presenter by suggesting alternative protocols, reagents, etc.  Sometimes the presenter will reveal data that was unanticipated, which is always interesting!  The lab meeting will conclude with a discussion on future goals that the presenter will hope to accomplish by the time s/he is scheduled to present again.  In our lab, I present about once a month, which is a reasonable amount of time to generate new data. I have a few experiments in progress and in about a week and a half, I will know if I will be going into the lab meeting with great data or so-so data. Until then, I am keeping my fingers crossed!

A must have book for young scientists

A fellow graduate student shared a book with me that I believe is invaluable to young research scientists.  At the bench, a laboratory navigator by Kathy Barker introduces readers to the laboratory environment, complete with discussions ranging from how labs are generally organized to laboratory safety and etiquette.

At the bench also describes how to properly setup experiments, carefully maintain your laboratory notebook and how to perform some important techniques commonly used in biological research.  In my opinion, two of the key topics in this book are the chapters that detail how you should present yourself and your data to other people.

I wish I had this book earlier in my career.  At the bench is written in an easy-to-read and understand manner such that it can be utilized by scientists as young as those that are in high school.  However, even as an advanced graduate student, I find the material in At the bench extremely useful.  The material is also easily accessible as this hard cover book is spiral bound so that when I am at the bench, I can quickly flip through pages for quick reference.  This book typically costs approximately $45.00, but consider it an investment in your career. You may even find it less expensive if you purchase a used copy.  Pass on the information because it is a great resource!

A summer of scientific research and teaching

There has been a lot going on during summer 2008. Over the past five weeks I served as a mentor for undergraduates in a summer research program hosted by my university. In this role, I assisted a small group of students with constructing scientific research posters and offered advice about the graduate school experience.


Just this past week I finished a course that counts toward receiving a certificate in college teaching. I decided to enroll in this certificate program because

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So much to do, too little time...

Where do the hours in the day go?? I began my experiments upon arrival into the lab this morning and before I knew it, it was 2 o’clock and I was not anywhere near finished with the first task on my list of experiments to perform today. Time in the lab just seems to vanish even faster when reading current research articles, having impromptu meetings with colleagues, and attending seminars and vendor fairs are added to the never-ending list of things to do. Each day, I juggle these activities within only eight hours. My time is quite stunted in the lab because I have to pick up my daughter from her after school program by 6pm everyday. And while I am in the lab, I can only focus on

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